Composite

Part:BBa_K4343080

Designed by: Xiner Liu   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-Scp2-BgElo9

BgElo9 gene encodes ∆-9 elongase from Blattella germanica. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.

Characterisation

We integrated BgElo9 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.

puc-huh-scp2-bgelo9-map.png

Result

BgElo9 was thought to have no preference towards C18:2. (Fig: Po1f-7)

part-6.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 7015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
    Illegal XhoI site found at 3465
    Illegal XhoI site found at 3498
    Illegal XhoI site found at 8012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3653
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 4706
    Illegal AgeI site found at 5067
    Illegal AgeI site found at 6209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 5247
    Illegal BsaI site found at 5784
    Illegal BsaI site found at 6212
    Illegal BsaI site found at 6739
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 6206
    Illegal BsaI.rc site found at 6268
    Illegal BsaI.rc site found at 7215
    Illegal BsaI.rc site found at 7485
    Illegal BsaI.rc site found at 8647
    Illegal SapI.rc site found at 1908
    Illegal SapI.rc site found at 4972


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